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Electrospray ionization interface to high pressure mass spectrometry and related methods

專利號
US10867781B2
公開日期
2020-12-15
申請人
The University of North Carolina at Chapel Hill(US NC Chapel Hill)
發(fā)明人
John Michael Ramsey; William McKay Gilliland, Jr.
IPC分類
H01J49/16; H01J49/24; H01J49/00; H01J49/04; B01L3/00
技術(shù)領(lǐng)域
mass,esi,analyzer,about,mm,inlet,chamber,ion,vacuum,trap
地域: NC NC Chapel Hill

摘要

An electrospray ionization (ESI)-mass spectrometer analysis systems include an ESI device with at least one emitter configured to electrospray ions and a mass spectrometer in fluid communication with the at least one emitter of the ESI device. The mass spectrometer includes a mass analyzer held in a vacuum chamber. The vacuum chamber is configured to have a high (background/gas) pressure of about 50 mTorr or greater during operation. During operation, the ESI device is configured to either; (a) electrospray ions into a spatial region external to the vacuum chamber and at atmospheric pressure, the spatial extent being adjacent to an inlet device attached to the vacuum chamber, the inlet device intakes the electrosprayed ions external to the vacuum chamber with the mass analyzer and discharges the ions into the vacuum chamber with the mass analyzer; or (b) electrospray ions directly into the vacuum chamber with the mass analyzer.

說明書

The internal end 15i of the inlet device 15 can be between about 1-50 mm from the front of the mass analyzer 30, e.g., the front endcap of the ion trap. In some embodiments, the internal end of the inlet device 15i can reside between about 1-10 mm or between about 1-5 mm from the front of the mass analyzer 30.

Although shown with the accelerating and gate electrode configurations in FIGS. 1 and 2 and with the focusing electrode 48 as a tube electrode 48t in FIGS. 3A and 4A, other focusing/lens electrode arrangements can be used. The discharge end of the inlet tube 15i can extend a distance into the focusing lenses and/or electrodes. For example, the focusing electrode 48 can comprise an Einzel lens and/or ion funnel 48f. FIGS. 3C and 4C illustrate that the mass spectrometer 10 can have a focusing electrode 48 that comprises an ion funnel electrode 48f upstream of the mass analyzer 30 in the vacuum chamber 12 holding the mass analyzer 30.

An accelerating electrode, such as electrode 28 (FIG. 1) is typically electrically connected to the capillary inlet tube 15 and/or capillary ESI device 20t, and the field generated accelerates ions toward the mass analyzer 30, e.g., ion trap. The “focusing electrodes” discussed above focus the ions (which may have been accelerated by the “accelerating electrode”) into the mass analyzer 30, e.g., ion trap. Thus, the mass spectrometer 10 can include a variety of different ion optic (focusing or “l(fā)ens” electrode configurations).

權(quán)利要求

1
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