A circuit board includes a substrate and two circuit layers. The substrate is provided with a conductive hole. The conductive hole is provided with a conductor. The substrate is provided with a groove on both sides, and each of the two circuit layers is respectively provided in the corresponding one of the two grooves. The groove includes a concave portion. The concave portion is located at the conductive hole, and the diameter of the concave portion is larger than the diameter of the conductive hole to expose a portion of the conductor. The circuit layer includes a connection pad located in the concave portion. The connection pad is shaped as a conductive protrusion, and the conductive hole electrically connects the two circuit layers. The manufacturing method of the circuit board of the present disclosure is relatively simple and the manufacturing cost is low. The circuit of the manufactured circuit board is formed in the groove, and the groove is formed by laser ablation. Therefore, the line width of the circuit layer is stable and the precision is high. The connection pad of the circuit board of the present disclosure is a conductive protrusion to improve the conductive yield.