Alternatively, optionally, as shown in FIG. 3, a material of the first pixel defining structure 31 is a lyophobic material, and a material of the second pixel defining structure 32 is a lyophilic material. The first pixel defining structure 31 is located on one side of the second pixel defining structure 32 away from the substrate 1.

The first pixel defining structure 31 and the second pixel defining structure 32 in the display substrate provided by the embodiments of the present disclosure are manufactured by adopting the hydrophobic material and the hydrophilic material respectively, so that the ink circulates in the pixel column openings ‘A’ advantageously and is not prone to overflowing into the adjacent pixel column opening A.

Optionally, as shown in FIG. 1 and FIG. 4-FIG. 7, in the display substrate provided by the embodiments of the present disclosure, the virtual pixel region 121 includes a first virtual pixel region 121a and a second virtual pixel region 121b located on one side of the display region 11 away from the first virtual pixel region 121a. Both the first virtual part 412 and the second virtual part 422 extend in the second direction X, the first virtual part 412 is in the first virtual pixel region 121a, and the second virtual part 422 is in the second virtual pixel region 121b.