Specifically, as shown in FIG. 4, the first color light-emitting layer 41, the second color light-emitting layer 42 and the third color light-emitting layer 43 respectively correspond to a red organic light-emitting layer R, a green organic light-emitting layer G and a blue organic light-emitting layer B. The blue light-emitting material is low in light-emitting efficiency and short in service life. Therefore, during specific implementation, a distance between first blocking walls 311 for limiting a blue pixel column may be designed to be greater than a distance between first blocking walls 311 for limiting a red pixel column, and greater than a distance between first blocking walls 311 for limiting a green pixel column, so that an area of the third main body part of the blue light-emitting layer is greater than an area of the first main body part of the red light-emitting layer, and greater than an area of the second main body part of the green light-emitting layer. That is, an area of a blue sub-pixel is greater than an area of a red sub-pixel, and greater than an area of a green sub-pixel, so as to compensate the light-emitting efficiency and service life of the blue light-emitting material.

According to the display substrate provided by embodiments of the present disclosure, the main body parts of adjacent organic light-emitting layers in the two colors are connected, thereby improving the uniformity of the thickness of the organic light-emitting layers advantageously.