At a first aspect, an embodiment of the present disclosure provides a display substrate, including sub-pixels with at least two different colors. The sub-pixels are arranged as pixel columns in a first direction, and all sub-pixels at the same pixel column have the same color. The display substrate includes a substrate, a first electrode layer, a pixel defining structure, and an organic light-emitting layer.

The substrate includes a display region and a bezel region around the display region.

The first electrode layer is on one side of the substrate and includes first electrodes in one-to-one correspondence with the sub-pixels.

The pixel defining structure includes a first pixel defining structure and a second pixel defining structure with a thickness being smaller than that of the first pixel defining structure. The first pixel defining structure includes first blocking walls in the display region and a second blocking wall in the bezel region. The first blocking wall extends in the first direction. Adjacent first blocking walls limit pixel column openings in one-to-one correspondence with the pixel columns. The second blocking wall extends in a second direction and is connected with first blocking walls limiting at least two adjacent pixel column openings of the same color. The second pixel defining structure includes third blocking walls in the display region. The third blocking walls are in the pixel column openings so as to divide the pixel column openings into pixel openings in one-to-one correspondence with the sub-pixels. An orthographic projection of one pixel opening on the substrate is within an orthographic projection of one first electrode on the substrate, and the second direction intersects with the first direction.