In the display substrate, all the main body parts of the light-emitting layers of the same color are connected, so that uniformity of the thicknesses of the light-emitting layers can be effectively improved. Arrangement of the pixel columns with the different colors for the case that all the main body parts of the light-emitting layers of the same color are connected is illustrated below in detail.

In some specific implementation, as shown in FIG. 6, the organic light-emitting layer further includes a third color light-emitting layer 43, and the third color light-emitting layer 43 includes third main body parts 431. In the second direction X, the first main body parts 411, the second main body parts 421 and the third main body parts 431 are arranged alternately.

Specifically, as shown in FIG. 6, the first color light-emitting layer 41, the second color light-emitting layer 42 and the third color light-emitting layer 43 respectively correspond to a red organic light-emitting layer R, a green organic light-emitting layer G and a blue organic light-emitting layer B. All the main body parts of the first color light-emitting layer 41 (the red organic light-emitting layer) and the second color light-emitting layer 42 (the green organic light-emitting layer) are connected through the corresponding virtual parts, while the third color light-emitting layer 43 (the blue organic light-emitting layer) only includes the third main body parts 431, and the third main body parts 431 are not connected. It should be noted that the main body parts of the light-emitting layers of any two colors may be connected according to the actual design situation.