In the display substrate as shown in FIG. 7, the plurality of third virtual parts 432 of the third color light-emitting layer 43 (the blue light-emitting layer) are located in the first virtual pixel region 121a or the second virtual pixel region 121b. For example, the third virtual parts 432 located in the first virtual pixel region 121a can achieve connection of the adjacent third main body parts 431 and will not increase a width of the first virtual pixel region 121a in the first direction Y.

As shown in FIG. 7, because the quantity of the blue pixel columns is large, the first blocking walls 311 limiting the red, green and blue pixel columns can be designed to be equidistant. That is, it can be considered that in the second direction X, the first main body part 411, the second main body part 421 and the third main body part 431 have the same width, thereby advantageously reducing the design difficulty of a mask for forming the first pixel defining structure 31 and reducing the difficulty of an etching process of the first pixel defining structure 31.

According to the display substrate provided by the embodiments of the present disclosure, the main body parts of the organic light-emitting layers of all the colors are completely connected respectively, so that ink of each color is communicated on the overall display substrate, thereby improving the uniformity of the thickness of the organic light-emitting layers advantageously.