The display substrate 10 further includes a plurality of cover plate alignment marks 14 configured for aligning the display substrate 10 with the cover plate 30. The plurality of cover plate alignment marks 14 are located in the frame region. By arranging the plurality of cover plate alignment marks 14 in the frame region, it is able to prevent the plurality of cover plate alignment marks 14 from affecting the display effect. In some embodiments, an orthographic projection of at least one of the plurality of cover plate alignment marks 14 on the base substrate is located between the orthographic projection of the dam glue 50 on the base substrate and the display region, that is, between the dam glue region and the display region (as illustrated by