For example, as illustrated in FIG. 1C, FIG. 1D and FIG. 2B, the display substrate 10 further includes an electrode layer extending portion 17 electrically connected with the light emitting element 12, and the electrode layer extending portion 17 is located in the frame region. For example, the electrode layer extending portion 17 is electrically connected with the second electrode layer 122 (e.g., the cathode) of the light emitting element 12, and is also electrically connected with the lead line 70 (as illustrated in FIG. 1H), so the second electrode layer 122 of the light emitting element 12 is electrically connected with the lead line 70 through the electrode layer extending portion 17. In the direction perpendicular to the base substrate 11, the electrode layer extending portion 17 is located between the cover plate alignment marks 14 (referring to FIG. 1C, FIG. 1D and FIG. 2B) and the base substrate 11; furthermore, an orthographic projection of the electrode layer extending portion 17 on the base substrate 11 is overlapped with the orthographic projections of the cover plate alignment marks 14 on the base substrate 11. Because the signal lines in the first frame region are densely distributed, by arranging the cover plate alignment marks 14 in the first frame region on a side of the electrode layer extending portion 17 away from the base substrate 11, the space of the first frame region can be effectively utilized without increasing the size of the frame region (that is, it is beneficial to achieve the narrow frame design).