Embodiments of the present disclosure further provide a manufacturing method of a display panel, which comprises: forming a pixel defining layer on a flexible base substrate; forming a plurality of light emitting elements on the base substrate, in which the pixel defining layer separates the plurality of light emitting elements; forming an encapsulation layer on the base substrate, in which the encapsulation layer is on a side of the plurality of light emitting elements away from the base substrate, and the encapsulation layer comprises a first inorganic encapsulation layer, a first organic encapsulation layer, and a second inorganic encapsulation layer which are sequentially arranged in a direction away from the base substrate; forming a plurality of cover plate alignment marks on the base substrate, in which the cover plate alignment marks are configured for aligning a display substrate comprising the pixel defining layer, the plurality of light emitting elements, the encapsulation layer, and the plurality of cover plate alignment marks with a cover plate; the display panel comprises a display region and a frame region at a periphery of the display region; the frame region comprises a dam glue region where an annular dam glue is located and an annular cutting region on a side of the dam glue away from the display region; at least one of the plurality of cover plate alignment marks is overlapped with the second inorganic encapsulation layer in a direction perpendicular to the base substrate, and an orthographic projection of the at least one of the plurality of cover plate alignment marks on the base substrate is between an orthographic projection of the dam glue on the base substrate and the display region, and/or an orthographic projection of at least one of the plurality of cover plate alignment marks on the base substrate is between the orthographic projection of the dam glue and the cutting region; forming an optical adhesive layer on a side of the plurality of cover plate alignment marks away from the base substrate; and connecting the display substrate formed with the plurality of cover plate alignment marks to the cover plate by using the optical adhesive layer, wherein the base substrate comprised in the display substrate is flat before connecting the display substrate to the cover plate and comprises a curved portion after connecting the display substrate to the cover plate.